学術論文 / Peer-Reviewed Journals


  1. M. Shinohara, H. Arakawa, Y. Oda, N. Shiraki, S. Sugiura, T. Nishiuchi, T. Satoh, K. Iino, S. Leo, Y. Kato, K. Araya, T. Kawanishi, T. Nakatsuji, M. Mitsuta, K. Inamura, T. Goto, K. Shinha, W. Nihei, K. Komori, M. Nishikawa, S. Kume, Y. Kato, T. Kanamori, Y. Sakai, H. Kimura,”Coculture with hiPS‑derived intestinal cells enhanced human hepatocyte functions in a pneumatic‑pressure‑driven two‑organ microphysiological system”Scientific reports,vol.11,5437,2021.[link]
  2. T. Matsumura, T. Sato, T. Abe, H. Sanjo, K. Katagiri, H. Kimura, T. Fujii, H. Tanaka, M. Hirabayashi, T. Ogawa,”Rat in vitro spermatogenesis promoted by chemical supplementations and oxygen-tension control”,Scientific reports,vol.11,3458,2021.[link]


  1. S. Mise, S. Shibagaki, S. Nishikawa, H. Nakamura, H. Kimura, *A. Takamatsu,”Diversity in self-organized forms and migration modes in isolated epithelial cells”,Artificial Life and Robotics,vol.25,pp523-528,2020.[link]
  2. Y. Ikeda, P. Jurica, H. Kimura, H. Takagi, Z.R. Struzik, K. Kiyono, Y. Arata, Y. Sako,”C. elegans episodic swimming is driven by multifractal kinetics”,Scientific Reports,vol.10,14775,2020.[link]
  3. K. Shinha, W. Nihei, *H. Kimura,”A microfluidic probe integrated device for spatiotemporal 3D chemical stimulation in cells”,Micromachines,vol.11,no.7,691,2020.[link]
  4. K. Shinha, W. Nihei, T. Ono, R. Nakazato, *H. Kimura,”A pharmacokinetic-pharmacodynamic model based on multi-organ-on-a-chip for drug-drug interaction studies”Biomicrofluidics,vol.14,no.4,44108,2020.[link]
  5. M. Chikamori, H. Kimura, R. Inagi, J. Zhou, M. Nangaku, *T. Fujii,”Intracellular calcium response of primary cilia of tubular cells to modulated shear stress under oxidative stress”,Biomicrofluidics,vol.14,no.4,44102,2020.[link]
  6. D. Kasahara, H. Sumiyoshi, H. Endo, T. Yanagawa, Y. Nakano, Y. Matsuki, S. Nakao, A. Kamiya, H. Kimura, *Y. Inagaki,”Visualization and Isolation of Zone-Specific Murine Hepatocytes That Maintain Distinct Cytochrome P450 Oxidase Expression in Primary Culture”Biochemical and Biophysical Research Communications ,vol.528,no.3,pp420-425,2020.[link]
  7. K. Sasaki, K. Bandai, M. Takatsuka, M. Fujii, M. Takagi, R. Kita, S. Yagihara, H. Kimura, N. Shinyashiki,”Heterogeneous solvent dielectric relaxation in polymer solutions of water and alcohols”,Frontiers in Physics,vol.8,no.84,2020.[link]
  8. H. Zhang, D. Sakagami, W. Huang, H. Kimura, Y. Okamura,”Measurement and modelling of tensile moduli of polymer blend thin films with phase separated structures”,Polymer,vol.190,122233,2020.[link]
  9. Arata Y, Oshima T, Ikeda Y, Kimura H, Sako Y,”OP50, a bacterial strain conventionally used as food for laboratory maintenance of C. elegans, is a biofilm formation defective mutant”,microPublication Biology,2020.[link]


  1. M. Komeya, H. Yamanaka, H. Sanjo, M. Yao, H. Nakamura, H. Kimura, T. Fujii, T. Sato, *T. Ogawa,”In vitro spermatogenesis in two-dimensionally spread mouse testis tissues”,Reproductive Medicine and Biology,vol.18,no.4,pp362-369,2019.[link]
  2. S. Yokoyama, A. Otomo, S. Hadano, *H. Kimura,”An open-type microdevice to improve the quality of fluorescence labeling for axonal transport analysis in neurons”,Biomicrofluidics,vol.13,no.3,34104,2019.[link]
  3. M. Nishikawa, S. Yuri, H. Kimura, N. Yanagawa, M. Hamon, P. Hauser, L. Zhao, OD Jo, N. Yanagawa,”Comprehensive analysis of chromatin signature and transcriptome uncovers functional lncRNAs expressed in nephron progenitor cells”,Biochimica et Biophysica Acta – Gene Regulatory Mechanisms,vol.1862,no.1,pp58-70,2019.[link]


  1. T. Ohkubo, H. Kinoshita, T. Maekawa, K. Kunida, H. Kimura, S. Kuroda, T. Fujii,”Development of a microfluidic cell culture and monitoring system for intracellular signaling studies”,bioRxiv,453100,2018.[link]
  2. T. Hiraiwa, Y. Nakai, T. G Yamada, R. Tanimoto, H. Kimura, Y. Matsumoto, N. Miki, N. Hiroi, A. Funahashi,”Quantitative analysis of sensitivity to a Wnt3a gradient in determination of the pole‐to‐pole axis of mitotic cells by using a microfluidic device”,FEBS Open Bio,vol.8,no.12,PP1920-1935,2018.[link]
  3. W. Huang, Y. Sunami*, H. Kimura, S. Zhang,”Applications of Nanosheets in Frontier Cellular Research”,Nanomaterials ,vol.8,no.7,519,2018.[link]
  4. K. Kojima, H. Nakamura, M. Komeya, H. Yamanaka, Y. Makino, Y. Okada, T. Sato, T. Fujii, H. Kimura, T. Ogawa,”Neonatal testis growth recreated in vitro by two-dimensional organ-spreading”,Biotechnology and Bioengineering,vol.115,no.12,pp3030-3041,2018[link]
  5. M. Nishikawa, H. Kimura, N. Yanagawa, M. Hamon, P. Hauser, L. Zhao, OD Jo, N. Yanagawa,”An optimal serum-free defined condition for in vitro culture of kidney organoids”,Biochemical and Biophysical Research Communications ,vol.501,no.4,pp996-1002,2018.[link]
  6. H. Kimura, M. Nishikawa, N. Yanagawa, H. Nakamura, S. Miyamoto, M. Hamon, P. Hauser, L. Zhao, O. D. Jo, M. Komeya, T. Ogawa, N. Yanagawa,”Effect of fluid shear stress on in vitro cultured ureteric bud cells in a pumpless microfluidic device”,Biomicrofluidics,vol.12,no.4,44107,2018.[link]
  7. H. Yamanaka, M. Komeya, H. Nakamura, H. Sanjo, T. Sato, M. Yao, H. Kimura, T. Fujii, T. Ogawa,”A monolayer microfluidic device supporting mouse spermatogenesis with improved visibility”,Biochemical and Biophysical Research Communications ,vol.500,no.4,pp885-891.2018.[link]
  8. K. Haneda, T. Oishi, H. Kimura, T. Inazu,”Development of a microbioreactor for glycoconjugate synthesis”,Bioorganic & Medicinal Chemistry,vol.26,no.8,pp2092-2098,2018.[link]
  9. H. Kimura, Y. Sakai, T. Fujii,”Organ/Body-on-a-chip Based on Microfluidic Technology for Drug Discovery”,Drug Metabolism and Pharmacokinetics,vol.33,no.1,pp43-48,2018.[link]


  1. M. Komeya, K. Hayashi, H. Nakamura, H. Yamanaka, H. Sanjo, K. Kojima, T. Sato, M. Yao, H. Kimura, T. Fujii, T. Ogawa,”Pumpless microfluidic system driven by hydrostatic pressure induces and maintains mouse spermatogenesis in vitro”,Scientific Reports,vol.7,no.1,15459,2017.[link]
  2. 高橋翼, 中村寛子, 木村啓志,”精子選別機能集積型受精卵作出デバイスの開発”,日本機械学会論文集,vol.83,no.850,16,2017.[link]
  3. 宮本隼佑,亀崎勇暁,諸星和,喜多理王,木村啓志,”ルードヴィヒ・ソレー効果を利用した重水分離システムの構築”,化学とマイクロ・ナノシステム学会会誌,vol.16,no.1,pp47-48,2017.
  4. Y. Nakano, S. Sachie, H. Sumiyoshi, K. Mikami, Y. Tanno, M. Sueoka, D. Kasahara, H. Kimura, T. Moro, A. Kamiya, K. Hozumi and Y. Inagaki,”Identification of a Novel Alpha-Fetoprotein-Expressing Cell Population Induced by Jagged1/Notch Signal in Murine Fibrotic Liver”,Hepatology Communications,vol.1,n0.3,pp.215-229,2017.[link]
  5. K. M. Ganesh, M. Morohoshi, Y. Yasoda, S. Yokoyama, H. Kimura, K. Tsuchiya,”ZnO Based Microfluidic pH Sensor: A Versatile Approach for Quick Recognition of Circulating Tumor Cells in Blood”,ACS Applied Materials & Interfaces,vol.9,no.6,PP5193-5203,2017.[link]


  1. Horayama, K. Shinha, K. Kabayama, T. Fujii, H. Kimura, “Spatial Chemical Stimulation Control in Microenvironment by Microfluidic Probe Integrated Device for Cell-Based Assay”, PLOS ONE, vol.11,no.12,2016.[link]
  2. M. Komeya, H. Kimura, H. Nakamura, T. Yokonishi, T. Sato, K. Kojima, K. Hayashi, K. Katagiri, H. Yamanaka, H. Sanjo, M. Yao, S. Kamimura, K. Inoue, N. Ogonuki, A. Ogura, T. Fujii, T. Ogawa, “Long-term ex vivo maintenance of testis tissues producing fertile sperm in a microfluidic device”, Scientific Reports, vol.6, 21472,  2016.[link]


  1. L. Yang, Y. Okamura, H. Kimura, “Surface Modification on Polydimethylsiloxane-based Microchannels with Fragmented Poly(L-lactic acid) Nanosheets”, Biomicrofluidics, vol.9, 064108, 2015.[link]
  2. H. Kimura, T. Ikeda, H. Nakayama, Y. Sakai, T. Fujii, “An On-chip Small Intestine-Liver Model for Pharmacokinetic Studies“, Journal of Laboratory Automations, vol.20, no.3, pp.265-273, 2015.[link]
  3. H. Tozawa, T. Maekawa, H. Kimura, T. Fujii, ” A novel effect of parylene-based surface coating on HepG2 cell function”, Materials Science and Engineering C, vol.46, pp.190-194, 2015.[link]


  1. P.V. Hauser, M. Nishikawa, H. Kimura, T. Fujii, N. Yanagawa, “Controlled Tubulogenesis from Dispersed Ureteric Bud-derived Cells Using a Micropatterned Gel”, Journal of Tissue Engineering and Regenerative Medicine, vol.10,no.9,pp762-771, 2014.[link]
  2. H. Nakayama, H. Kimura, T. Fujii, Y. Sakai, “Image-based evaluations of distribution and cytotoxicity of Irinotecan (CPT-11) in a multi-compartment micro-cell coculture device”, J. Biosci. Bioeng., vol.117(6), pp. 756–762, 2014.[link]
  3. M. Shinohara, H. Kimura, K. Montagne, K. Komori, T. Fujii, Y. Sakai, “Combination of microwell structures and direct oxygenation enables efficient and size-regulated aggregate formation of an insulin-secreting pancreatic beta-cell line”, Biotechnol. Prog., vol.30(1), pp.178-187, 2014.[link]


  1. X. He, H. Kimura, T. Fujii, “A High-Throughput Device for Patterned Differentiation of Embryoid Bodies”, Journal of Robotics and Mechatronics, vol.25, no.4, pp.623-630, 2013.[link]


  1. J. Kawada, H. Kimura, H. Akutsu, Y. Sakai, T. Fujii, “Spatiotemporally controlled delivery of soluble factors for stem cell differentiation”, Lab on a Chip, vol.12, no.21, pp.4508-4515, 2012.[link]
  2. M.M. Chowdhury, H. Kimura, T. Fujii, Y. Sakai, “Induction of alternative fate other than default neuronal fate of embryonic stem cells in a membrane-based two-chambered microbioreactor by cell-secreted BMP4”, Biomicrofluidics, vol.6,014117, 2012
  3. K. Komori, S. Fujii, K. Montagne, H. Nakamura, H. Kimura, T. Otake, T. Fujii, Y. Sakai “Development of a Well-of-the-Well System-Based Embryo Culture Plate with an Oxygen Sensing Photoluminescent Probe”, Sensors and Actuators B, vol.162, pp.278-283, 2012
  4. T. Nojima, S. Kaneda, H. Kimura, T. Yamamoto, T. Fujii, “Application of Cell-free Expression of GFP for Evaluation of Microsystems”, Frontiers in Bioscience, vol.17, pp.1931-1939, 2012


  1. Y. Nakao, H. Kimura, Y. Sakai, T. Fujii,” Bile canaliculi formation by aligning rat primary hepatocyte in a microfluidic device”, Biomicrofluidics, vol.5, pp.022212-1-7, 2011
  2. T. Kurakazu, K. Kuribayashi-Shigetomi, Y. T. Matsunaga, H. Kimura, T. Fujii, Y. Sakai, S. Takeuchi, “Selective Retrieval Method Using Electrolytically Generated Bubbles for Cell Array Applications”, Sensors and Actuators B, vol.159, pp.229-233, 2011


  1. H. Kimura, H. Takeyama, K. Komori, T. Yamamoto, Y. Sakai, T. Fujii, “Microfluidic Device with Integrated Glucose Sensor for Cell-based Assay in Toxicology”, Journal of Robotics and Mechatronics, vol.22, no.5, pp.594-600, 2010[link]
  2. 木村啓志,庄野裕基, Pereira-Rodrigues, 山本貴富喜,酒井康行,藤井輝夫,“オンチップグルコースセンサによる細胞活性オンライン計測の検討”,電気学会論文誌E,vol.130, no.10, pp. 476-483, 2010.[link]
  3. M.M. Chowdhury, T. Katsuda, K. Montagne, H. Kimura, K. Nobuhiko, H. Akutsu, T. Ochiya, T. Fujii, Y. Sakai, ” Enhanced effects of secreted soluble factor preserve better pluripotent state of embryonic stem cell culture in a membrane-based compartmentalized micro-bioreactor”, Biomed Microdevices., vol.12, no.6, pp1097-1105, 2010.[link]


  1. H. Kimura, H. Nakamura, T. Akai, T. Yamamoto, H. Hattori, Y. Sakai, T. Fujii, “On-chip Individual Embryo Coculture with Microporous Membrane-supported Endometrial Cells”, IEEE Transactions on NanoBioscience, vol.8, no.4, pp.318-324, 2009(Chemical Biology (2008, issue 6)にも掲載).[link]
  2. 木村啓志,中山秀謹,山本貴富喜,酒井康行,藤井輝夫,“体内毒性試験へ向けたオンチップ小腸-肝臓由来細胞共培養システムの開発”,電気学会論文誌E,129, no.8, pp. 252-258, 2009.[link]
  3. 木村啓志,中村寛子,岩井孝介,山本貴富喜,竹内昌治,藤井輝夫,酒井康行,“ダイナミックマイクロアレイを用いた受精卵操作の自動化の試み”,電気学会論文誌E,129, no.8, pp. 245-251, 2009.[link]


  1. T. Nojima, T. Yamamoto, H. Kimura, T. Fujii, “Polymerase chain reaction-based biochemical logic gate coupled with cell-free transcription/ translation of green fluorescent protein as a report gate”, Chemical Communications, vol.28, no.32, pp.3771-3773, 2008.[link]
  2. T. Nojima, H. Kimura, T. Fujii, “Cell-free Protein Synthesis Conducted by Template DNA with Repetitive Sequence”, Chemistry Letters, vol.37, no.6, pp.648-649, 2008.[link]
  3. H. Kimura, T. Yamamoto, H. Sakai, Y. Sakai, T. Fujii, “An Integrated Microfluidic System for Long-term Perfusion Culture and On-line Monitoring of Intestinal Tissue Models”, Lab on a Chip, vol.8, no.5, pp.741-746, 2008, May.[link]


  1. H. Kimura, M. Nishikawa, T. Yamamoto, Y. Sakai, T. Fujii, “Microfluidic Perfusion Culture of Human Hepatocytes”, Journal of Robotics and Mechatronics, vol.19, no.5, pp.550-556, 2007.[link]
  2. H. Nakayama, H. Kimura, K. Komori, T. Fujii Y. Sakai, “Development of a Multi-Compartment Micro-Cell Culture Device as a Future on-Chip Human: Fabrication of a Three-Compartment Device and Immobilization of Rat Mature Adipocytes for the Evaluation of Chemical Distributions”, Journal of Robotics and Mechatronics, vol.19, no.5, pp.544-549, 2007.[link]

書籍・総説など / Books and Review Papers

  1. 木村啓志,酒井康行,藤井輝夫,“創薬を加速するツールとしてのOrgan-on-a-chipの進展”,薬剤学,vol.76, No.4, pp.238-242, 2016年7月
  2. 木村啓志,酒井康行,藤井輝夫,“マイクロ流体デバイス技術によるOrgan/Body-on-a-chipの実現に向けた取り組み”,月刊「細胞」,ニュー・サイエンス社, vol.48, no.3, pp.148-151, 2016年2月
  3. 木村啓志,酒井康行,藤井輝夫,“第4章:医療計測用バイオマテリアル,第13節:マイクロ流体デバイス技術を応用したin vitro生体モデルの構築”,先端バイオマテリアルハンドブック,エヌティーエス出版,pp.474-477, 2012年6月15日発刊, 書籍分担執筆
  4. 木村啓志,山本貴富喜,竹内昌治,酒井康行,藤井輝夫,“第5章:バイオチップの将来技術,第9節:機能集積型マイクロ流体デバイスの細胞培養への応用”,バイオチップ実用化ハンドブック,エヌティーエス出版,pp.557-563, 2010年4月書籍分担執筆
  5. 木村啓志,竹内昌治,酒井康行,藤井輝夫,“マイクロ流体技術を応用した受精卵培養システム”,バイオインダストリー, シーエムシー出版,vol.27, no.2, pp.43-49,2010年2月
  6. 木村啓志,竹内昌治,金範埈,酒井康行,藤井輝夫,藤田博之,“細胞操作技術の最前線:第3回細胞選択技術”,Medical Bio,オーム社,vol.6, no.4, pp.8-10,2009年6月
  7. 西川昌輝,中山秀謹,木村啓志,山本貴富喜,藤井輝夫,酒井康行,“化学物質の体内動態を再現する複合細胞培養系の開発と利用”,バイオインダストリー,シーエムシー出版,vol.25,no.1,pp.17-25,2008年1月
  8. 木村啓志,酒井康行,藤井輝夫,“集積型マイクロ流体デバイスによるヒト臓器由来細胞の機能解析”,細胞工学,学研メディカル秀潤社,vol.26,no.12,pp.1435-1440,2007年12月
  9. 木村啓志,酒井康行,藤井輝夫,“膜を有する細胞培養デバイスとその応用”,機能材料,シーエムシー出版,vol.27, no.5, pp.62-68,2007年5月
  10. 木村啓志,酒井康行,藤井輝夫,“第4章:細胞の3D組織化,第7節:集積化マイクロ灌流培養デバイスによる臓器由来細胞の培養と計測”, 動物実験代替のためのバイオマテリアル・デバイス,シーエムシー出版210-220,2007年8月 書籍分担執筆


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